VWR International 1325F Manual do Utilizador

0WENGKECEKFRWTKƂECVKQP/QFKH[KPIGP\[OGU2%44GCNVKOG2%4'NGEVTQRJQTGUKU%NQPKPI6TCPUHGEVKQP'NGEVTQRQTCVKQP&GVGEVKQP894+06'40#6+10

Nucleic Acid PurificationEndotoxin-Free Plasmid Isolation8www.vwr.comDescription Pk Cat. No.PerfectPrep™ EndoFree Plasmid Maxi kit, 10 preps 10 Tests7

ElectrophoresisReagents & Buffers98www.vwr.comDescription Pk Cat. No.Agarose I™ 100 g0710-100GAgarose I™ 250 g0710-250GAgarose I™ 500 g0710-500GAg

99All You Need GenomicsElectrophoresisReagents & BuffersDescription Pk Cat. No.Agarose gel loading dye, 6X, containing 3 tracking dyes and 15% Fic

ElectrophoresisReagents & Buffers100www.vwr.comDelivering High Quality & Superior PerformancePlease contact your local VWR sales office orgo to

101All You Need GenomicsElectrophoresisMarkers & DyesDescription Pk Cat. No.100 bp ladder, sufficient for 50 lanes 250 µlK180-250UL100 bp Ready La

ElectrophoresisMarkers & Dyes102www.vwr.comDescription Pk Cat. No.DNA MicroMarker™, sufficient for 50 lanes 100 µGE840-100UGDescription Pk Cat. No

103All You Need GenomicsElectrophoresisMarkers & DyesDescription Pk Cat. No.EZ-Vision® 1 DNA dye kit, with dye migrating at ~10 bp in a 1% agarose

ElectrophoresisMarkers & Dyes104www.vwr.comVWR.COMTALKING YOUR LANGUAGEThe local website with global reachDescription Pk Cat. No.GelRed™ 3X in wat

105All You Need GenomicsElectrophoresisGel TanksBuffer tank volume (ml) 250Capacity 1 large gel (12,5×13 cm), 2 small gels (12,5×6 cm) or 4 micro gels

ElectrophoresisGel Tanks106www.vwr.comBuffer volume (ml) 325Gel size W×L (mm) 60×75Sample capacity 32Unit W×L×H (mm) 130×240×65Recommended running vol

107All You Need GenomicsElectrophoresisGel TanksBuffer volume (ml) 450Gel size W×L (mm) 100×115Sample capacity 80Unit W×L×H (mm) 165×230×65Recommended

9All You Need GenomicsNucleic Acid PurificationFastfilter Plasmid IsolationDescription Pk Cat. No.FastPlasmid Mini kit, 100 preps 100 Tests733-0175Fas

ElectrophoresisGel Tanks108www.vwr.comBuffer volume (ml) 900Gel size W×L (mm) 128×150Sample capacity 112Unit W×L×H (mm) 200×320×70Recommended running

109All You Need GenomicsElectrophoresisGel TanksBuffer volume (ml) 1200Gel size W×L (mm) 150×150Sample capacity 120Unit W×L×H (mm) 215×335×70Recommend

ElectrophoresisGel Tanks110www.vwr.comBuffer volume (ml) 2200Gel size W×L (mm) 200×200Sample capacity 168Unit W×L×H (mm) 270×475×80Recommended running

111All You Need GenomicsElectrophoresisGel TanksBuffer volume (ml) 3000Gel size W×L (mm) 250×300Sample capacity 624Unit W×L×H (mm) 330×560×90Recommend

ElectrophoresisGel Tanks112www.vwr.comModel Mini 10 Midi Plus 10 Midi Plus 15Buffer volume (ml) 225 300 500Gel size W×L (mm) 70×100 150×100 150×150Sam

113All You Need GenomicsElectrophoresisPower SuppliesModel 300 V 250 VOutput voltage range/increments (V) 2 - 300/1 5 - 250/1Output current range/incr

PCR Cloning 116Cloning

CloningPCR Cloning116www.vwr.comPk Cat. No.40 AssaysPRME2201310Order your free copy online at http://eu.vwr.com/chemicalTHE CHEMICAL BRANDFROM VWR INT

N_pj\kkc\]fiZcfe\jn`k_c`kkc\mXc`[Xk`fe6:_ffj\Kil\FI=>fc[%<ogi\jj`femXc`[Xk\[YpN\jk\ieKiXej]\Zk`fei\X[p<Xj`cpj_lkkc\[`ekf-

Nucleic Acid PurificationGenomic DNA from Blood10www.vwr.comDescription Pk Cat. No.ArchivePure™ DNA Blood kit, for 30 ml blood 1KIT733-1014ArchivePure

RWTG+RTGEKUG+RGTHQTOCPEG(QTTGUGCTEJKP&0#40#2TQVGKP%GNN%WNVWTGCPF*KUVQNQI[GZRGEVUWRGTKQTRGTHQTOCPEGHTQO#/4'5%1oUHCOKN[QH

Reagents 120Transfection

TransfectionReagents120www.vwr.comDescription Pk Cat. No.jetPRIME® DNA and siRNA transfection reagent, 0,75 ml, with 60 ml jetPRIME® buffer 0,75 mlPPL

121All You Need GenomicsTransfectionReagentsDescription Pk Cat. No.jetPEI®-FluoF DNA transfection reagent, 0,5 ml, with 50 ml NaCl solution 150 mM 500

TransfectionReagents122www.vwr.comDescription Pk Cat. No.jetPEI®-FluoR DNA transfection reagent, 0,5 ml, with 50 ml NaCl solution 150 mM 500 µlPPLU106

123All You Need GenomicsTransfectionReagentsINSTRUMENT SERVICEAND MAINTENANCEPlease contact your local VWR sales office for more informationHotlineRepa

#..;170''&(14.+37+&*#0&.+0)4GSWGUVQWTCEEQORCP[KPIRWDNKECVKQPn#NN[QWPGGFHQT.KSWKF*CPFNKPIo(NCUMUCPF%[NKPFGTU2KRGV

Reagents 126Electroporators, Cuvettes & Accessories 127Electroporation

ElectroporationReagents126www.vwr.comE-BUSINESSMore than just a webshop for all of your laboratory needs Please visit us at: http://eu.vwr.com/e-busin

127All You Need GenomicsElectroporationElectroporators, Cuvettes & AccessoriesInterfaces Digital user interfaceCharge time 5 seconds maximumVoltag

11All You Need GenomicsNucleic Acid PurificationGenomic DNA from BloodDescription Pk Cat. No.Ready PCR DNA column kit, 50 preps 50 Tests733-1050Descri

ElectroporationElectroporators, Cuvettes & Accessories128www.vwr.comInterfaces Digital user interfaceCharge time 5 seconds maximumVoltagerangeLowV

129All You Need GenomicsElectroporationElectroporators, Cuvettes & AccessoriesDescription Pk Cat. No.HT 96 with ECM 830 generator, HT 200 plate ha

ElectroporationElectroporators, Cuvettes & Accessories130www.vwr.comAC parameters (alignment)Frequency (MHz) 1, fixedVoltage (VRMS) 0 - 75Duration

131All You Need GenomicsElectroporationElectroporators, Cuvettes & AccessoriesDescription Pk Cat. No.In vivoGenetrode™, straight, 5 mm* 1 732-0010

ElectroporationElectroporators, Cuvettes & Accessories132www.vwr.comDescription Pk Cat. No.Tweezertrode™ electrode*, 7 mm diameter 1732-0015Tweeze

133All You Need GenomicsElectroporationElectroporators, Cuvettes & AccessoriesParameter Optimisation chamber Production chamberVolume 2 ml 9 mlOut

ElectroporationElectroporators, Cuvettes & Accessories134www.vwr.comDelivering High Quality & Superior PerformancePlease contact your local VW

Gel Documentation 136Spectrophotometers & Accessories 138Detection

DetectionGel Documentation136www.vwr.comDescription Pk Cat. No.GenoMini gel documentation system 1730-3002Description Pk Cat. No.GenoSmart2 gel docume

137All You Need GenomicsDetectionGel DocumentationVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachDescription Pk Cat. No.GenoPlex CHEMI gel

Nucleic Acid PurificationGenomic DNA from Plant12www.vwr.comINSTRUMENT SERVICEAND MAINTENANCEPlease contact your local VWR sales office for more inform

DetectionSpectrophotometers & Accessories138www.vwr.comLight source Long-life stabilised xenon flash lampDetector Twin CCD arrayWavelength range (

139All You Need GenomicsDetectionSpectrophotometers & AccessoriesModel Multiskan GO without cuvette port Multiskan GO with cuvette portLight sourc

www.vwr.comTable of contents140Standard High Purity Plasmid Isolation ... 6Endotoxin Free Plasmid Isolation ... 8(CUVƂNVGT

)161894%1/(146*'.#6'560'9552'%+#.1(('45#0&&'6#+.51(;174.1%#.894&+564+$7614<241894+&

13All You Need GenomicsNucleic Acid PurificationGenomic DNA from Tissue and CellsDescription Pk Cat. No.illustra™ triplePrep kit, 50 preps 50 Tests28-

Nucleic Acid PurificationGenomic DNA from Tissue and Cells14www.vwr.comDescription Pk Cat. No.ArchivePure™ DNA Cell/Tissue kit, for 1g tissue or 2×10⁸

15All You Need GenomicsNucleic Acid PurificationGenomic DNA from Yeast and BacteriaDescription Pk Cat. No.ArchivePure™ DNA Yeast and Gram-positive Bac

Nucleic Acid PurificationGenomic DNA from Soil, Stool and Water16www.vwr.comDescription Sample size Pk Cat. No.E.Z.N.A.® Soil DNA Mini kit 1 g 50 Test

17All You Need GenomicsNucleic Acid PurificationGenomic DNA from FungiVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachDescription Average yi

8KUKVQWTFGEKECVGFOKETQUKVGUQPXYTEQOHQTCHWNNTCPIGQH.KHG5EKGPEG 4GUGCTEJCPF&GXGNQROGPV 5CHGV[CPF%NGCPKPI1WTYGDUKVGEQPVCKPUCNN

Nucleic Acid PurificationGenomic DNA from Insect and Mollusc18www.vwr.comE-BUSINESSMore than just a webshop for all of your laboratory needs Please vi

19All You Need GenomicsNucleic Acid PurificationGenomic DNA PreparationDescription Pk Cat. No.illustra™ Genomiphi™ HY kit, 25 reactions 1KIT25-6600-22

Nucleic Acid PurificationRNA from Blood20www.vwr.comDescription Sample size Pk Cat. No.PerfectPure™ RNA Blood kit 0,05 - 3 ml whole blood and buffy co

21All You Need GenomicsNucleic Acid PurificationmiRNA/mRNA IsolationKit QuickPrep™ Micro mRNA purification kit QuickPrep™ mRNA purification kitNo. of

Nucleic Acid PurificationmiRNA/mRNA Isolation22www.vwr.comDescription Pk Cat. No.illustra mRNA Purification Kit, 2 purifications (4 columns) 1KIT27-92

23All You Need GenomicsNucleic Acid PurificationRNA from Cells and Soft TissueDescription Sample size Pk Cat. No.PerfectPure™ RNA Cultured Cell kit 10

Nucleic Acid PurificationRNA from Cells and Soft Tissue24www.vwr.comDescription Pk Cat. No.illustra™ RNAspin Mini isolation kit, 20 preps 20 Tests25-0

25All You Need GenomicsNucleic Acid PurificationRNA from Fatty TissueVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachAverage yield Binding c

Nucleic Acid PurificationRNA from Fribous Tissue26www.vwr.comDescription Sample size Pk Cat. No.PerfectPure™ RNA Fibrous Tissue kit 0,5 - 40 mg fibrou

27All You Need GenomicsNucleic Acid PurificationTotal RNA ReagentsDescription Pk Cat. No.Isol-RNA Lysis Reagent™, 200 ml 200 ml733-1089Description Pk

)'01/+%51.76+1056TCPUHGEVKQP'NGEVTQRQTCVKQP6TCPUHGEVKQPTGCIGPVU'NGEVTQRQTCVKQPEWXGVVGU'NGEVTQRQTCVQTU5CORNGRTGR2NCUOKFRWTK

Nucleic Acid PurificationTotal RNA Reagents28www.vwr.comPLG HeavyAqueous phase High density sample (for example, high salt content)Organic phaseStanda

29All You Need GenomicsNucleic Acid PurificationRNA StabilisationDescription Pk Cat. No.RNase-free DNase set, 50 reactions 50 Tests733-1093E-BUSINESSM

Nucleic Acid PurificationPCR Clean Up30www.vwr.comDescription Pk Cat. No.PCRExtract Mini kit, 50 preps 50 Tests733-2071PCRExtract Mini kit, 200 preps

31All You Need GenomicsNucleic Acid PurificationPCR Clean UpDescription Pk Cat. No.illustra™ ExoStar™ 1-STEP kit 100 TestsUS77702illustra™ ExoStar™ 1-

Nucleic Acid PurificationDNA/RNA Gel Extraction32www.vwr.comDescription Pk Cat. No.Agarose GelExtract Mini kit, 50 preps 50 Tests733-1988Agarose GelEx

33All You Need GenomicsNucleic Acid PurificationDNA/RNA Clean UpDescription Pk Cat. No.illustra™ ProbeQuant™ G-50 Micro Columns 5028-9034-08Descriptio

Nucleic Acid PurificationDye Terminator Removal34www.vwr.comDescription Pk Cat. No.illustra AutoSeq™ G-50 columns 25027-5340-02Description Pk Cat. No.

35All You Need GenomicsNucleic Acid PurificationDNA StorageDescription Pk Cat. No.FTA® CardsFTA® Classic Card 25512-1160FTA® Classic Card 100512-1079F

Nucleic Acid PurificationDNA Storage36www.vwr.com512-1154Description Pk Cat. No.FTA® Elute Micro Card, 4 sample areas per card 25730-1364FTA® Elute Mi

37All You Need GenomicsNucleic Acid PurificationDNA StorageDelivering High Qualit y & Superior Per formancePlease contact your local VWR sales offi

*$,,+L6FDQ640L6HT62/L' 62/L' ,RQ7RUUHQW*6)/; *6-U$UH\RXGRLQJ1H[W*HQ6HTXHQFLQJ"+HUH¶V DQRWKHU WLS IRU \RXVLP

© 2011 Thermo Fisher Scientific Inc. All rights reserved. You shouldn’t have to choose between purity and flexibility. Sensitivity and productivity. Thr

DNA/RNA Modifying Enzymes 40Modifying Enzymes

Modifying EnzymesDNA/RNA Modifying Enzymes40www.vwr.comDescription Pk Cat. No.DNA polymerase I, Klenow fragment fromE. coli200 EU A5217.0200DNA polyme

41All You Need GenomicsModifying EnzymesDNA/RNA Modifying EnzymesVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachDescription Pk Cat. No.Prot

RWTG+RTGEKUG+RGTHQTOCPEG(QTTGUGCTEJKP&0#40#2TQVGKP%GNN%WNVWTGCPF*KUVQNQI[GZRGEVUWRGTKQTRGTHQTOCPEGHTQO#/4'5%1oUHCOKN[QH

Standard PCR Reagents 44 Hot Start PCR Reagents 49 Direct PCR Reagents 52 High Fidelity PCR Reagents 53Long Range PCR Reagents 56dNTPs and Buffer

PCRStandard PCR Reagents44www.vwr.comDescription Pk Cat. No.TaqDNA polymerase, 100 U (40 - 100 reactions of 50 µl) 100 733-0105TaqDNA polymerase, 250

45All You Need GenomicsPCRStandard PCR reagentsDescription Pk Cat. No.TaqDNA polymerase (native, without BSA), 200 units (5 U/µl) 1KIT FERMEP0281TaqDN

PCRStandard PCR reagents46www.vwr.comDescription Pk Cat. No.DreamTaq™ PCR master mix (2X), 4×1,25 ml (for 200 reactions of 50 µl) 5 mlFERMK1071DreamTa

47All You Need GenomicsPCRStandard PCR reagentsDescription Pk Cat. No.illustra™ PuReTaq Ready-To-Go™ PCR Beads (0,2 ml tubes/multiwell plate) 96 Tests

'HVFULSWLRQ ,OOXPLQD 5RFKH /LIH7HFK,QVWUXPHQW6\VWHPV*HQRPH$QDO\]HU,,H,,[+L6HT0L6HT+L6FDQ64*6)/;*6-XQLRU 62/L' ,RQ7RUU

PCRStandard PCR reagents48www.vwr.comDescription Pk Cat. No.MasterTaq kit, 250 U (100-250 reactions of 50 µl) 250733-0114MasterTaq kit, 500 U (200-500

49All You Need GenomicsPCRHot Start PCR ReagentsDescription Pk Cat. No.PerfectTaq™ Plus DNA polymerase, sufficient for 40×50 µl reactions 50 EU733-206

PCRHot Start PCR reagents50www.vwr.comDescription Pk Cat. No.Maxima™ Hot StartTaqDNA polymerase, 100 units (5 U/µl) 1KIT FERMEP0601Maxima™ Hot StartTa

51All You Need GenomicsPCRHot Start PCR reagentsDescription Pk Cat. No.illustra™ Hot Start Master Mix, 100 reactions 1KIT25-1500-01Description Pk Cat.

PCRDirect PCR Reagents52www.vwr.comDescription Pk Cat. No.PCR directly from plantsPhire® Plant Direct PCR kit, 200 reactions (50 µl each) 1KITADVAF-13

53All You Need GenomicsPCRHigh Fidelity PCR ReagentsDescription Pk Cat. No.Exact polymerase, sufficient for 40 reactions 100 EU733-0993Exact polymeras

PCRHigh Fidelity PCR reagents54www.vwr.comDescription Pk Cat. No.PfuDNA polymerase (recombinant), 100 units (2,5 U/µl) 1KIT FERMEP0501PfuDNA polymeras

55All You Need GenomicsPCRHigh Fidelity PCR reagentsDescription Pk Cat. No.Phusion® High-Fidelity PCR kit, 50×50 µl reactions 1KITFINRF-553SPhusion® H

PCRLong Range PCR Reagents56www.vwr.comDescription Pk Cat. No.PCR Extender system, 100 U (50 - 100 reactions) 100733-0189PCR Extender system, 200 U (1

57All You Need GenomicsPCRdNTPs and BuffersDescription Pk Cat. No.dNTP Mix, 200 µl (10 mM) 200 µl733-0130dNTP Mix, 1000 µl (10 mM) 1ml733-0131Descript

'HVFULSWLRQ ,OOXPLQD 5RFKH /LIH7HFK,QVWUXPHQW6\VWHPV*HQRPH$QDO\]HU,,H,,[+L6HT0L6HT+L6FDQ64*6)/;*6-XQLRU 62/L' ,RQ7RUU

PCRdNTPs and Buffers58www.vwr.comDescription Pk Cat. No.10XTaqbuffer with Mg2+,1,8ml 1,8 ml 733-0122Description Pk Cat. No.5X TaqMaster PCR-Enhancer 1

+LJKTXDOLW\7UXH+3/&SXUL¿HG&XVWRP2OLJRV3ULPHUವ2QOLQHRUGHUSODWIRUPYLDZZZYZUFRP/LIH6FLHQFHRUZZZWKHUPRFRPROLJRRUGHUವ

#..;170''&)7+&'5*CPF[TGHGTGPEGVQQNUHQT[QWTCRRNKECVKQPQTVGEJPKSWG4GSWGUV[QWTEQR[HTQO[QWTNQECN894UCNGUQHƂEG$KQ$

All You Need GenomicsPCRTubes61VWR offer a complete range of PCR tubes.Here we have made a selection for you of the most popular tubes. The plastic ar

www.vwr.comPCRStrips62PCR stripsECNDescription Pack size Profile Type of strips Cap ColourTubes & caps per strip732-4800 Abgene Strip of 8 PCR tub

All You Need GenomicsPCRCaps63Here we have made a selection for you of the most popular caps for the PCR strips tubes. The plastic are designed, manuf

www.vwr.comPCRPlatesPCR plates compatibility tableChoosing the right PCR plate for your PCR cycler and assay is important.WůĂƚĞŵŽĚĞůƌĞĐŽŵŵĞŶĚĂƟŽŶƐĂ

All You Need GenomicsPCRPlatesApplied Biosystems / Life Technologies Biorad/MJ Eppendorf Stratagene Biometra Roche Veriti™ 3847000/ 7300/7500/7700/790

www.vwr.comPCRPlatesThermo ScientificApplied Biosystems / Life TechnologiesCat.No. Description Profile ColourNumber of wells SkirtedPiko 24Piko 96Arkt

All You Need GenomicsPCRPlatesApplied Biosystems / Life Technologies Biorad/MJ Eppendorf Stratagene Biometra RocheVeriti™ 3847000/ 7300/7500/7700/7900

Standard High Purity Plasmid Isolation 6Endotoxin Free Plasmid Isolation 8(CUVƂNVGT2NCUOKF+UQNCVKQP Genomic DNA from Blood 10Genomic DNA from

www.vwr.comPCRSeals68PCR sealsHere we have made a selection for you of the most popular PCR seals. PCR seal platesECNDescription pack size732-0498 Abg

#..;170''&(14.+37+&*#0&.+0)4GSWGUVQWTCEEQORCP[KPIRWDNKECVKQPn#NN[QWPGGFHQT.KSWKF*CPFNKPIo(NCUMUCPF%[NKPFGTU2KRGV

PCRHeat Sealers70www.vwr.comOperating humidity range (%) 20 - 80% non condensingOperating temperature range (°C) 18...30Temperature set range (°C) 125

71All You Need GenomicsPCRHeat SealersVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachAir input pressure (psi/bar) 80 psi (5,5 bar) - 87 psi

PCRPCR Workstations72www.vwr.comModel UV3 Cabinet UV3 WorkstationFilterPre-filterCarbon filterHEPA filterZerOzone catalyst gridPre-filterCarbon filter

73All You Need GenomicsPCRPCR CyclersModel ³Prime ³PrimeX ³PrimeGBlock accuracy (°C) <±0,25* <±0,25* <±0,25*Block homogeneity (°C) <±0,3*

PCRPCR Cyclers74www.vwr.com732-0663 732-1021Model Prime PrimeGBlock accuracy (°C) <±0,2* <±0,2*Block homogeneity (°C) <±0,3* <±0,3*Gradien

75All You Need GenomicsPCRPCR CyclersE-BUSINESSMore than just a webshop for all of your laboratory needs Please visit us at: http://eu.vwr.com/e-busin

PCRPCR Cyclers76www.vwr.comOrder your free copy online at http://eu.vwr.com/chemicalTHE CHEMICAL BRANDFROM VWR INTERNATIONALModel TCP0024 TCP0096Block

cDNA Synthesis 78SYBR Green qPCR 82TaqMan Probe qPCR 86One -Step TaqMan qRT-PCR 89One -Step SYBR qRT-PCR 89Reference Gene Assays 90HRM 91Hydrol

Nucleic Acid PurificationStandard High Purity Plasmid Isolation6www.vwr.comDescription Pk Cat. No.PerfectPrep™ Spin Mini kit, 50 preps 50 Tests733-206

Real Time PCRcDNA Synthesis78www.vwr.comDescription Pk Cat. No.Masterscript kit, 50 reactions 50 Tests733-0108Description Pk Cat. No.DyNAmo™ cDNA Synt

79All You Need GenomicsReal Time PCRcDNA SynthesisDescription Pk Cat. No.M-MuLV Reverse Transcriptase, 5000 units (20 U/µl) 1KITFERMEP0351Activity, %T

Real Time PCRcDNA Synthesis80www.vwr.comDescription Pk Cat. No.Ready-To-Go™ T-Primed First-Strand kit 50 Tests27-9263-01Description Pk Cat. No.Ready-T

81All You Need GenomicsReal Time PCRcDNA SynthesisDescription Pk Cat. No.Phusion® RT-PCR kit, 20 reactions 1KITFINRF-546SPhusion® RT-PCR kit, 100 reac

Real Time PCRSYBR Green qPCR82www.vwr.comINSTRUMENT SERVICEAND MAINTENANCEPlease contact your local VWR sales office for more informationHotlineRepairM

83All You Need GenomicsReal Time PCRSYBR Green qPCRDelivering High Quality & Superior PerformancePlease contact your local VWR sales office orgo to

Real Time PCRSYBR Green qPCR84www.vwr.comVWR.COMTALKING YOUR LANGUAGEThe local website with global reachDescription Pk Cat. No.DyNAmo™ Flash SYBR® Gre

85All You Need GenomicsReal Time PCRSYBR Green qPCRdR/dT0.30.60.91.21.51.82.1858075706590Temperature (°C)Fluorescence (norm)00.20.40.60.81.01.21.44 8

Real Time PCRTaqMan Probe qPCR86www.vwr.comDescriptionPkCat. No.PerfeCTa® qPCR FastMix® II without ROXPerfeCTa® qPCR FastMix II®, 250×20 µl reactions

87All You Need GenomicsReal Time PCRTaqMan Probe qPCRDescription Pk Cat. No.qPCR SuperMix, 50 reactions50Assays733-1272qPCR SuperMix, 200 reactions200

7All You Need GenomicsNucleic Acid PurificationStandard High Purity Plasmid IsolationDescription Pk Cat. No.illustra™ plasmidPrep Midi Flow kit, 25 pr

Real Time PCRTaqMan Probe qPCR88www.vwr.comDescription Pk Cat. No.DyNAmo™ Flash Probe qPCR kit, 40×50 µl reactions 1 KITFINRF-455SDyNAmo™ Flash Probe

89All You Need GenomicsReal Time PCROne -Step TaqMan/SYBR qRT-PCRDescription Pk Cat. No.One Step Fast qRT-PCR kits without passive reference dyeOne-St

Real Time PCRReference Gene Assays90www.vwr.comDescription Pk Cat. No.ACTB PerfeCTa® reference gene assay, Human ACTB (β-actin), 500×20 ul reactions 5

91All You Need GenomicsReal Time PCRHRMDescription Pk Cat. No.AccuMelt™ HRM SuperMix (2×1,25 ml), sufficient for 250×20 µl reactions 2,5 mlQUNT95103-2

Real Time PCRHydrolysis Probe Genotyping92www.vwr.comDescription Pk Cat. No.AccuStart™ Genotyping Toughmix® without ROXAccuStart™ qPCR ToughMix®, 250×

93All You Need GenomicsReal Time PCRmicroRNA ProfilingDescription Pk Cat. No.qScript™ microRNA cDNA synthesis kit, 25×20 µl reactions 25 Assays733-201

Real Time PCRqPCR Cyclers94www.vwr.comThermal blockBlock formats 24-well, 96-well (not interchangeable)Sample volume 10 - 50 µl (PikoReal® 24); 5 - 20

95All You Need GenomicsReal Time PCRqPCR CyclersDescription Pk Cat. No.Piko® plate illuminator 1731-0358INSTRUMENT SERVICEAND MAINTENANCEPlease contac

#..;170''&)7+&'5*CPF[TGHGTGPEGVQQNUHQT[QWTCRRNKECVKQPQTVGEJPKSWG4GSWGUV[QWTEQR[HTQO[QWTNQECN894UCNGUQHƂEG$KQ$

Reagents & Buffers 98Markers & Dyes 101Gel Tanks 105Power Supplies 113Electrophoresis